Journal:

Article Title: Differential Contribution of IL-4 and STAT6 vs STAT4 to the Development of Lupus Nephritis 1

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Figure Lengend Snippet: Treatment of NZM.2410 mice with an anti-IL-4 mAb inhibits progression of renal disease, but does not diminish IgG anti-dsDNA Ab production. Twenty-week-old NZM.2410 mice were treated with either 11B11, GL113, or PBS, as described in Results (n = 11–12 mice per group). A, Effect of 11B11 treatment on proteinuria. Data are represented as percentage of cumulative prevalence of severe (≥300 mg/dl) proteinuria. B, Kidneys harvested from treated and control mice were sectioned and scored. Glomerular and tubulointerstitial activity and chronic lesion scores were summed to obtain a total KBS, which is shown as individual sample scores. C, Comparison of individual mouse KBS representing the components of glomerular activity (glomerular cellularity score) and chronic glomerular lesions (glomerulosclerosis score) in treated and control mice. D, Representative H&E (upper panels)- and Jones silver-stained (lower panels) kidney sections are shown. GH, glomerular hypercellularity; GSc, glomerulosclerosis; Lo, glomerular lobulation; SL, segmental lesion; DT, dilated tubules. E, Serum IgG anti-dsDNA Ab levels (p < 0.05, 11B11-treated vs GL113 or PBS controls at 5 mo of age). F, Protein extracts from the kidneys of 11B11- or GL113-treated mice were tested for total IgG, IgG1, and IgG2a by ELISA. Results are expressed as the mean ± SE of Ig in pg/mg of protein in the kidney extracts (p = NS; n = 4–6 per group). Similar results were obtained in another experiment in which 6- to 8-wk-old NZM.2410 were treated with 11B11 or GL113 for 16 wk.

Article Snippet: Measurement of serum IgE levels Serum IgE levels were measured using flat-bottom white high-binding ELISA plates (ISC Bioexpress), which were coated with 50 μ l of anti-mouse IgE mAb (EM-95; 10 μ g/ml in 0.1 M Tris-HCl, pH 8.3) overnight at 4°C.

Techniques: Control, Activity Assay, Comparison, Staining, Enzyme-linked Immunosorbent Assay